FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37579034
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17800.021.2344745
CGCCAAT24000.015.46869732
CTCGTAT24650.015.15201841
CGTATGC25350.014.91083443
GTCACGC25700.014.88333628
TCTCGTA24150.014.44090740
TCGTATG27150.014.00514242
ACGCCAA28800.013.35945231
AGTCACG30200.012.4421127
ACACGTC30800.012.12588812
TATCTCG28250.011.94681438
CGTCTGA32750.011.81606115
ATCTCGT30300.011.58407339
ACGTCTG36650.010.80424114
ATCGGAA35200.010.7448471
TCGGAAG37800.010.596712
CACGTCT41550.09.58424413
CGGAAGA43250.09.1573423
GATCGGA22800.08.4917511
GCACACG50700.07.632571710
Produced by FastQC (version 0.11.5)