FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27429070
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA16100.014.9531741
CGTATGC17550.014.74323944
CTCGTAT16900.014.37835742
TCGTATG20300.012.74600243
TATGCCG7700.012.56420245
ATCTCGT20550.011.93415440
GTCACGT22850.011.81623529
AGTCACG22150.011.78333928
CGTCTGA25550.010.39110516
CACGTGA32050.09.056128531
AATCTCG30250.08.99988539
ACACGTC28350.08.96800813
ACGTGAA30300.08.7622832
GATCGGA27750.08.7611481
TCACGTG29900.08.72906530
CGTGAAA29700.08.71202633
ACGTCTG31500.08.49976715
ATCGGAA32700.07.91395472
TCGGAAG32300.07.8726223
CGGAAGA36450.07.22322274
Produced by FastQC (version 0.11.5)