FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28619670
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7800.08.07634745
TCTCGTA10001.024091E-96.07505540
CTCGTAT10952.3283064E-105.95894841
TCGTATG12901.8189894E-125.9300942
CGTATGC11802.564775E-105.720200543
CGAAGAT22550.05.69064471
TACTCGG92500.05.618901335
CTACTCG95800.05.40186234
TCGTATA15805.456968E-125.268685311
AGACGGA85200.05.12392142
GAGACGG186350.05.1223541
GTAGAGA325050.05.08368971
Produced by FastQC (version 0.11.5)