FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25325328
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8250.014.99914445
CTCGTAT10050.012.08938741
TCTCGTA10300.012.01444640
CGTATGC12400.09.79815643
TCGTATG12800.09.66774142
ATCTCGT14900.08.90930939
CGTCTGA16100.08.52444215
ATCGGAA15250.08.2668171
ACACGTC17750.07.478549512
TCGGAAG17900.07.4171692
ACGTCTG21850.06.693047514
CACGTCT26050.05.873042613
CGGAAGA22450.05.6132043
AATCTCG29950.05.18357938
CGTAGAA10101.5991209E-65.12656641
GGACGAA6600.00149860445.11643551
Produced by FastQC (version 0.11.5)