FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005329851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005329851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9824056
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4500.026.99775345
CTCGTAT6550.018.20616741
TCTCGTA6300.017.85729240
CGATCAG7350.017.44876932
CGTATGC7350.016.5301643
GTCACGA7600.015.9866528
TCGTATG7950.015.28260142
ACGATCA8450.014.37859931
ATCTCGT8050.014.25477739
AGTCACG9550.013.90035627
TCACGAT9500.013.73674729
ACACGTC10300.012.01392912
ATCGGAA11300.011.1600221
CGTCTGA11300.010.95074915
ACGTCTG12700.010.27504914
TCGGAAG12650.010.1396632
CACGTCT13800.09.29298913
CACGATC16200.09.02771630
CGGAAGA13500.09.0011753
GTATGCC14400.08.4372744
Produced by FastQC (version 0.11.5)