FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12204
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5174.236315961979678Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2502.0485086856768273Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG630.5162241887905604Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG370.3031792854801704Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGTCGCCG160.13110455588331696Illumina Single End PCR Primer 1 (96% over 29bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC254.1045678E-795.9211659
AGAGCGG301.214994E-679.934318
CGGAAGA1800.066.6119164
AAGAGCG1850.064.811597
GATCGGA1850.064.811591
GAAGAGC1850.064.811596
TCGGAAG1850.064.811593
ATCGGAA1850.064.811592
AGATCGG602.6193447E-1063.9474451
GAGCGTC1600.062.948269
AGAGCGT1600.062.948268
TTGGCAG250.00471812857.55278
GGAAGAG2250.053.2895365
TAGGGGA451.9645086E-1047.76239818-19
GGGTCGC452.0372681E-1047.56584542-43
CGGGGGT452.0372681E-1047.56584538-39
GGGGTCG452.0372681E-1047.56584540-41
CGGCGGG205.2481366E-447.5658412-13
GATCATT205.2481366E-447.5658450-51
TCGGGGG600.047.5658438-39
Produced by FastQC (version 0.11.5)