FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73539
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT9921.3489440976896612TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC5890.8009355580032363TruSeq Adapter, Index 2 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC4560.620079141679925TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG4370.5942425107765947TruSeq Adapter, Index 2 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG3530.4800174057302928TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA3030.4120262717741606TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA1930.26244577707067TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT890.12102421844191516TruSeq Adapter, Index 18 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACT27700.045.0225792-93
TCACTGA27650.044.93215694-95
GTCACTG27800.044.8606294-95
TCACGTC3700.040.44730492-93
ACGTCCG3750.039.90800594-95
GGATCGG250.001604264138.03351676-77
CATGGGT250.001615037337.9817710-11
CACGTTG250.001615037337.9817780-81
CACGTCC3900.037.76398594-95
CAATCTC1500.036.39919738-39
CAGTCAC35350.035.41375492-93
CGATGTA1300.034.69488534-35
ACCAATC1450.034.38004736-37
CCAGTCA37700.033.08024290-91
TCCAGTC37950.032.9875190-91
CTCCAGT37900.032.6549788-89
AACTCCA37500.032.6121186-87
ACCGATG1400.032.2166832-33
GAACTCC38050.032.2031286-87
GAAACGA2150.032.01951236-37
Produced by FastQC (version 0.11.5)