FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73539
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC13911.8915133466595957Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5890.8009355580032363Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1560.21213233794313224Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG1180.16045907613647178Illumina Single End PCR Primer 1 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC700.074.9015359
GCGGGCA200.00203814271.4969256
ATCGGGA408.731331E-871.4969252
GATCGGG451.9732579E-763.5528221
AGAGCGG900.063.5528228
CGGGAGA551.1314114E-860.664064
TGATCTC250.004934526557.197544
GAGAGCG405.264417E-447.6646167
CCGGATC301.4898033E-647.4700748-49
GGCGCCG650.047.47006644-45
CGCCGGA404.2055035E-947.47006646-47
CGCCGTA3050.042.8008846-47
GGGTCGC402.2558015E-741.5363142-43
CCGTATC3200.041.5363148-49
GGGAGAG1059.658834E-1040.8553855
GCCGGAT354.3031414E-640.6886346-47
AGCGGCG653.6379788E-1240.26284410-11
GGGCGCC850.039.09342-43
GTATCAT3500.038.65419850-51
TCGGGGG1600.038.56942738-39
Produced by FastQC (version 0.11.5)