FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38630
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC11803.0546207610665284Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3560.9215635516438001Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG1250.3235827077400984Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCG610.157908361377168Illumina Single End PCR Primer 1 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA353.1286618E-1095.214292
GATCGGG409.0403773E-1083.312491
GAGCGGC505.287802E-966.659
AGAGCGG505.287802E-966.658
TCGGGAG551.123226E-860.5909083
GAGAGCG407.310089E-659.5089267
GTCTCAC250.004940332457.1285746
AGAATTA250.004940332457.1285741
GAGGGGC451.46880575E-552.8968249
CGGGAGA902.0918378E-1047.6071434
TCGGGTA252.7972706E-547.51458414-15
GATCATT357.813833E-847.4838150-51
GTCGGGT252.8080194E-547.4838112-13
CCGGATC404.149115E-947.48380748-49
GCCGGAT404.149115E-947.48380746-47
CGTCGGG205.3658284E-447.48380712-13
CGCCGGA301.4775924E-647.48380746-47
GCGTCGG205.3658284E-447.48380710-11
TCGGGGG1200.045.50531438-39
CTCGGGG1000.045.1096236-37
Produced by FastQC (version 0.11.5)