FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2874412
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC52335318.20730639866519TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG40277614.012465853885942TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATGC97740.34003476189217136TruSeq Adapter, Index 5 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATATCGTATG79620.27699578209386827TruSeq Adapter, Index 5 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCGATCTCGTATGC50460.17554894705421492TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGAGATCTCGTATGC35700.12419931450327928TruSeq Adapter, Index 5 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG940600.046.353811
CCGTCTT1071850.045.90572450-51
TGCTTGA1014550.045.90261558-59
CTTGAAA988150.045.8796460-61
TGCCGTC1078200.045.74967648-49
TCTGCTT1077450.045.61363256-57
TATGCCG1093900.045.56418646-47
CTTCTGC1081050.045.4463654-55
GTCTTCT1080350.045.41489452-53
CGTATGC1103150.045.25744644-45
CTCGTAT1087250.044.92142542-43
TGAAAAA981750.044.91882362-63
ATCTCGT1083800.044.5526340-41
TGATCTC1083600.043.6847838-39
AGTGATC1084800.043.45854636-37
ACAGTGA1147850.042.54815334-35
ACACAGT1161900.042.1068632-33
TCACACA1205400.041.31762330-31
AGTCACA1218400.041.0048128-29
CCAGTCA1230150.040.62858226-27
Produced by FastQC (version 0.11.5)