FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519863
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC693162.750784467250799Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC26380.10468823106653022Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA48400.082.613072
CGGGAGA86500.077.4627464
TCGGGAG74250.076.108383
GTCGGGA41050.073.34292
GAGCGGC53800.069.368449
GATCGGG60050.068.483651
ATCCGGA9100.067.839712
CCGGAGA19200.066.532384
GAGGGGC123600.065.843679
CGGGAAA33950.064.762444
GTCCGGA10150.064.0968252
CGGAGAG30100.063.8942685
TCGGGAA23550.062.712123
TCCGGAG14400.062.327733
GTTCGGA8650.062.036082
CCGGAAA12950.060.8723874
AGAGCGG68850.060.7566268
GTCGGAA15500.059.7427142
GACGGGA27200.057.613872
ACGGGAG25550.057.4314273
Produced by FastQC (version 0.11.5)