FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26702821
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG107700.022.88570246-47
CGTATGC110550.022.23149344-45
CTCGTAT109400.022.07486242-43
ATCTCGT132350.018.24699640-41
TGCCGTC151450.016.4154648-49
CCGTCTT155750.015.88596850-51
CGTCTGA198100.014.40136916-17
TCGTATG109900.013.78510344-45
CACGTCT224900.012.82775514-15
TCTCGTA116800.012.56434942-43
ATGCCGT128200.011.77998448-49
CACACGT249300.011.67766612-13
GCCGTCT143800.010.55154350-51
CGATGTA64100.010.44666634-35
ACCAATC119950.010.27454736-37
GTATGCC156050.010.07341646-47
AATCTCG85750.09.91387240-41
TCTGAAC311650.09.645205518-19
CGGAAGA496350.09.47281194-95
ATCGGAA546850.09.08003594-95
Produced by FastQC (version 0.11.5)