FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15658998
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG170420.10883199550826943TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG88950.023.83912846-47
CGTATGC89300.023.77258944-45
CTCGTAT92550.022.52802342-43
ATCTCGT107600.019.31085240-41
TGCCGTC110450.019.24127648-49
CCGTCTT115800.018.39327450-51
TCGTATG90300.015.25213544-45
ATGCCGT98800.013.67514248-49
TCTCGTA98200.013.61450642-43
ACCAATC116800.013.33728236-37
GCCGTCT108450.012.52395950-51
GTATGCC114250.012.03392346-47
AGTCACT228900.011.9408194-95
AATCTCG100100.011.62432340-41
CTTGAAA195550.010.92879760-61
CGATGTA21800.010.67484134-35
CGTCTTC131900.010.53140352-53
CAGTCAC275150.010.46876894-95
ACGTCTG262650.010.46077282-83
CGTCTGA250200.010.44979582-83
Produced by FastQC (version 0.11.5)