FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18252383
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT905540.49612152013246713TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA508330.2785006209874075TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG229710.1258520599748537TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC224500.12299763817141028TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG199850.10949255228755611TruSeq Adapter, Index 7 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG376250.028.05301948-49
CGTATGC381800.027.72638746-47
CTCGTAT374150.027.46837644-45
AGTCAAC275750.026.98952534-35
AACAATC270450.026.56925638-39
TGCCGTC399000.026.35691550-51
ATCTCGT388050.026.28892742-43
CCGTCTT401200.026.27171152-53
CCAGTCA630000.023.48926526-27
CAATCTC321500.022.63834240-41
ACAGTCA342600.021.99380732-33
TCGTATG384000.021.86616144-45
ATGCCGT385950.021.52768348-49
TCAACAA350900.021.43267836-37
TCTCGTA378950.021.34317642-43
TCACAGT390900.021.33638230-31
CTTGAAA489600.021.19946962-63
GCCGTCT395100.021.10015150-51
CTCCAGT709100.020.93937324-25
AATCTCG303650.020.77064140-41
Produced by FastQC (version 0.11.5)