FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15861317
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT387930.24457615972242405TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA196110.1236404265799618TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA192990.12167337680723486TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG171030.10782837263765677TruSeq Adapter, Index 7 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG165760.1045058238228263TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC159820.10076086367859617TruSeq Adapter, Index 12 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG261000.023.77314248-49
CGTATGC264550.023.54418646-47
CTCGTAT264200.023.00913844-45
TGCCGTC270700.022.74497850-51
CCGTCTT277250.022.35340352-53
AGTCAAC143300.022.22253234-35
ATCTCGT289050.020.85875542-43
AACAATC150150.020.5597638-39
ATGCCGT264700.018.72217248-49
TCGTATG269500.018.63565844-45
GCCGTCT266900.018.57604250-51
AATCTCG180900.018.39069740-41
AGTCACC213950.018.3826328-29
TCTCGTA270050.017.96481942-43
GTATGCC289600.017.35043146-47
CGTCTTC287800.017.33444452-53
CCAGTCA561700.017.33134826-27
CTTGAAA353550.017.28813462-63
ACAGTCA195400.016.60144832-33
TGCTTGA383050.016.30389660-61
Produced by FastQC (version 0.11.5)