FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15861317
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC808630.5098126467051884Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA165300.032.56246-47
CCGTATC170950.031.68164648-49
GTCGCCG178350.031.04379544-45
CGTATCA161800.028.80668850-51
GCCGTAT166250.028.03562248-49
TGGTCGC189800.027.53221342-43
TCGCCGT166200.027.48567646-47
GATCGGG32750.027.0589581
GTATCAT200450.026.8769550-51
ATCGGGA33200.025.9643942
GGTCGCC189050.024.76564244-45
TCGGTGG241750.023.70838-39
ATCATTA254350.023.14175852-53
GTGGTCG207900.022.14321142-43
TCTCGGT256450.021.34030336-37
CATTAAA302100.020.62295754-55
CGGTGGT235600.019.72148940-41
GGTGGTC278100.019.0809840-41
CTCGGTG251300.019.05625538-39
TATCATT250100.019.02547852-53
Produced by FastQC (version 0.11.5)