FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12698705
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC514380.40506492591173665Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC116250.029.3908948-49
CGTATCA110400.026.28131550-51
GCCGTAT116050.025.00178348-49
GTATCAT146200.023.3537450-51
GATCGGG27850.023.2755171
ATCGGGA27650.021.7090782
ATCATTA192850.019.85902452-53
TCGCCGT186400.019.028994-95
CGCCGTA179650.018.96550846-47
CATTAAA220700.018.53606854-55
TATCATT183400.016.1439752-53
CGCCGGA17950.016.13745946-47
GTCGCCG283750.016.03129694-95
TCATTAA198150.015.88854354-55
TGGTCGC288550.015.63297292-93
GGTCGCC295350.015.02385394-95
GAGCGGC43700.014.9251479
GTGGTCG300050.014.69356892-93
GGCGCCG31700.014.0802744-45
AGAGCGG47200.013.9205928
Produced by FastQC (version 0.11.5)