FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37060053
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA5261071.4196067123811182TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT3521350.9501740324008711TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1229950.037.9300748-49
TGCCGTC1237150.037.42051750-51
CCGTCTT1237250.037.36904552-53
CGTATGC1258050.037.16739746-47
CTCGTAT1282600.036.43428844-45
ATCTCGT1321500.035.07405542-43
CCGCACA1424050.032.5705636-37
TGCTTGA1383300.032.44416460-61
GTCCGCA1442200.032.12288734-35
CTTGAAA1406850.031.45745862-63
ACGTCCG1485700.031.36302832-33
GCACATC1480300.031.07296238-39
GTCTTCT1537000.030.2897154-55
ACATCTC1555200.029.82287640-41
TCACGTC1589550.029.46858230-31
TCTGCTT1605950.029.22467258-59
CTTCTGC1620300.028.8626456-57
AGTCACG1638900.028.68446728-29
CCAGTCA1889500.025.02588726-27
TGAAAAA1788050.024.61025264-65
Produced by FastQC (version 0.11.5)