FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20806399
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT216490210.404981659728817TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA5899322.835339262695097TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTAT973220.4677503300787416TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATATCGTA282470.13576111849051822TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA4242100.058.0386479
AGAGCAC4258750.057.821778
CGGAAGA4317450.057.463954
TCGGAAG4338600.057.305293
ATCGGAA4365350.056.9816172
GAAGAGC4360550.056.7120976
GATCGGA4392600.056.7103421
AAGAGCA4370600.056.4578557
GGAAGAG4568350.054.3014565
TATGCCG3562200.043.63010848-49
CCGTCTT3536100.043.50492552-53
TGCCGTC3550350.043.47629550-51
CGTATGC3580550.043.47406846-47
CTCGTAT3420600.043.31339344-45
ATCTCGT3403150.042.97312542-43
AACGATC3377000.042.81425538-39
CTTGAAA3427300.042.57053862-63
TGCTTGA3504100.042.48341460-61
CGATCTC3430000.042.3826540-41
GAAACGA3650550.042.27029836-37
Produced by FastQC (version 0.11.5)