FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20806399
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5705642.7422525156803923Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCC240310.11549812151540495Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA242300.080.267922
GATCGGG269850.073.884571
CGGGAGA340750.071.979714
GAGCGGC247900.067.542259
AGAGCGG272200.066.237618
TCGGGAG322650.065.735833
GTCGGGA107400.061.2024732
GAGAGCG184300.055.262167
CGGGAAA137100.053.0674484
TCGGGAA100900.051.457613
GAGGGGC477400.050.6109129
CGGAGAG127200.048.840045
GTCCGGA37450.045.2418022
ACGGGAG119250.044.932343
CCGGAGA86750.044.3685074
GAACGGG99600.043.361621
ATCCGGA53050.043.2995572
AAGCGGC82400.043.1397939
GTTCGGA31600.042.8036962
GATCCGA30300.041.507511
Produced by FastQC (version 0.11.5)