FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45459478
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA347550.030.34191346-47
CCGTATC365400.029.0487948-49
GTCGCCG388800.027.80792844-45
CGTATCA347050.026.20326850-51
GCCGTAT353550.025.80189148-49
TGGTCGC395900.025.5559442-43
TCGCCGT354250.024.90315246-47
GTATCAT466850.022.62276650-51
GGTCGCC420150.022.02222344-45
GATCGGG90750.021.955621
TCGGTGG536050.021.5598338-39
GTGGTCG419450.021.30226542-43
ATCGGGA90550.020.9314252
TCTCGGT523150.020.83998136-37
ATCATTA632050.018.82601552-53
GATCTCG652400.018.34314334-35
CGGTGGT496200.018.29456540-41
CATTAAA758250.016.85012254-55
CTCGGTG565900.016.819138-39
GGTGGTC619500.016.80625340-41
Produced by FastQC (version 0.11.5)