FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39623206
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1172480.2959074033534793Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA293450.026.42393946-47
CCGTATC311500.024.96189348-49
GTCGCCG322000.024.95045344-45
TGGTCGC322300.023.07863442-43
GCCGTAT304900.021.80343248-49
CGTATCA304900.021.7726450-51
TCGCCGT298200.021.7673946-47
AGAGCGT635800.021.4950549
GATCGGG71150.020.095641
AAGAGCG702450.019.598368
GGTCGCC343000.019.5882844-45
ATCGGGA73900.019.3381082
GTGGTCG346800.019.01792742-43
TCGGTGG460200.017.99966238-39
GTATCAT433250.017.99134650-51
GATCTCG494000.017.51743134-35
ATCGGAA799100.017.2846183
TCGGAAG800700.017.2605114
AGATCGG814150.017.081911
CGGAAGA805600.017.0761665
Produced by FastQC (version 0.11.5)