FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29414780
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC898300.3053906913463232Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA156150.035.08070446-47
GCCGTAT160300.033.53878448-49
CGTATCA160950.033.1972950-51
CCGTATC166750.032.98201448-49
AGAGCGT347800.032.0473449
TCGCCGT165000.031.60174846-47
GTCGCCG180550.031.21743444-45
TGGTCGC185600.029.25598142-43
AAGAGCG387200.029.0852588
GGTCGCC199000.027.49994544-45
GTGGTCG202400.026.510942-43
ATCGGAA439750.025.7020023
GATCGGA452950.025.0292072
TCGGAAG456650.024.7655334
CGGAAGA461250.024.373875
TCGGTGG242350.023.8070138-39
AGATCGG477250.023.7140941
CGGTGGT231650.023.37938740-41
TCTCGGT244850.023.04949836-37
GTATCAT244900.022.69988850-51
Produced by FastQC (version 0.11.5)