FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42314086
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC822300.19433244995531748Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT158350.031.86752148-49
CGTATCA157750.031.79341350-51
CCGTATC169550.030.14060248-49
CGCCGTA174700.029.3167946-47
TCGCCGT189350.025.80710246-47
GTCGCCG208950.024.90620444-45
TGGTCGC212100.023.67525942-43
AGAGCGT449150.023.4601659
GGTCGCC231000.022.07657444-45
AAGAGCG499900.021.2817768
GTGGTCG238300.021.10216142-43
GTATCAT269500.019.2797450-51
TCTCGGT278100.019.1836536-37
ATCGGAA559850.019.0563413
GATCGGA571850.018.6832432
TCGGTGG295700.018.42769438-39
GATCTCG304250.018.22003634-35
TCGGAAG590950.018.0411684
CGGTGGT286050.017.82079940-41
AGATCGG604100.017.676981
Produced by FastQC (version 0.11.5)