FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13281432
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC551120.41495525482493156Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA93500.033.95281246-47
CGTATCA93450.033.18638250-51
GCCGTAT95350.032.62458848-49
CCGTATC98250.032.33835648-49
GTCGCCG102750.031.3087744-45
TCGCCGT97900.031.16566746-47
TGGTCGC102850.030.44840642-43
GGTCGCC110700.028.52414144-45
AGAGCGT224650.028.358489
GTGGTCG112550.027.6765842-43
TCTCGGT123000.026.2326736-37
TCGGTGG128800.025.40215338-39
GTATCAT127050.025.32565750-51
CGGTGGT129000.024.44249340-41
AAGAGCG267400.023.8983368
GATCTCG144100.023.60833734-35
TAGATCT139850.023.51096732-33
CTCGGTG139900.023.03028538-39
TCGTGTA142100.022.37891414-15
CGTGTAG148300.021.0626616-17
Produced by FastQC (version 0.11.5)