FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15480916
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT566760.36610236758600073TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA403050.260352811164404TruSeq Adapter, Index 13 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG236700.031.24017348-49
CGTATGC240700.030.73088346-47
CTCGTAT236900.030.27157244-45
TGCCGTC255900.028.87768650-51
ATCTCGT248750.028.60056542-43
CCGTCTT258800.028.56327252-53
CCGTATC38200.022.25488738-39
AACAATC220450.022.22259938-39
AACGATC39650.022.1597238-39
AGTCACG61700.021.97814428-29
CTTGAAA330300.021.89850262-63
AGTCAAC237250.021.13919834-35
TGCTTGA365050.020.25626460-61
GTCTTCT385250.019.6626154-55
AGTTCCG44700.019.39037334-35
TTCCGTA43950.019.23520536-37
TCGTATG241000.019.2333944-45
ATGCCGT245600.018.87315648-49
CAATCTC265100.018.8469840-41
TATCTCG34900.018.71126642-43
Produced by FastQC (version 0.11.5)