FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13601689
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC511910.3763576714627132Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA114800.029.491746-47
CCGTATC121200.028.0520748-49
GTCGCCG127500.027.29844944-45
CGTATCA115850.025.35164650-51
GCCGTAT117200.025.22123348-49
TCGCCGT118350.024.47449546-47
TGGTCGC134250.024.21056442-43
ATCGGGA26500.022.2888722
GTATCAT153150.022.10726750-51
GGTCGCC136750.021.8754744-45
GATCGGG28400.021.4797781
TCGGTGG177550.019.99111738-39
GTGGTCG147600.019.83318542-43
ATCATTA194600.018.96010452-53
TCTCGGT179100.018.78394536-37
CGGTGGT169950.017.19703140-41
GATCTCG220200.016.88417434-35
CATTAAA238300.016.6484854-55
CTCGGTG183700.016.50434538-39
GGTGGTC209950.015.71865840-41
Produced by FastQC (version 0.11.5)