FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20917904
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1337700.6395000187399272TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1143080.5464601042245916TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG416100.032.68081746-47
CGTATGC433150.031.75157744-45
CTCGTAT438350.031.11921342-43
CCGTCTT425950.031.09065450-51
TGCCGTC430600.030.84723748-49
ATCTCGT464800.029.3529240-41
ACCAATC531750.026.0084236-37
TGCTTGA488900.025.26204758-59
CTTGAAA500500.024.47673660-61
CAATCTC581250.024.09643438-39
GTCTTCT579750.023.07597452-53
TGACCAA634650.022.9064134-35
ACTGACC654000.022.39930232-33
TCTGCTT628050.021.76294356-57
CTTCTGC659000.020.66526454-55
AGTCACT718150.020.52710728-29
CCAGTCA744500.019.8420926-27
TCACTGA753100.019.6046330-31
TGAAAAA645200.019.2744862-63
CGTCTGA799300.018.61230516-17
Produced by FastQC (version 0.11.5)