FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20917904
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1187670.5677767715159224Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA261100.026.60432446-47
CCGTATC262300.026.38770348-49
CGTATCA253400.026.3155150-51
GCCGTAT259850.025.79553648-49
GTCGCCG283050.024.6581244-45
TCGCCGT266600.024.64009346-47
TGGTCGC292250.022.88305742-43
ATCGGGA44850.022.6496412
GATCGGG45900.022.6486591
GGTCGCC298500.022.56296344-45
GTATCAT312250.022.13999250-51
GTGGTCG312500.021.01293442-43
ATCATTA371950.019.87111352-53
TCGGTGG362850.019.79759238-39
CGGTGGT345000.019.23291640-41
TCTCGGT363300.019.15241636-37
CATTAAA420850.018.55078954-55
GATCTCG410500.018.24520734-35
TATCATT371900.018.17981552-53
TCATTAA388200.017.930954-55
Produced by FastQC (version 0.11.5)