FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18365664
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT31150.06.30205192-93
CGTATCA28700.06.03546994-95
CGCCGTA30050.05.841182794-95
GTCGCCG48100.05.52184894-95
TCGCCGT41200.05.21335694-95
GATCTCG130600.05.1107794-95
TGGTCGC54600.05.075984594-95
Produced by FastQC (version 0.11.5)