FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14258076
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC417380.29273234341014875Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA94850.031.56452446-47
CCGTATC103350.029.15254248-49
GTCGCCG109600.028.50640344-45
TGGTCGC108550.026.35498242-43
CGTATCA96800.026.29327850-51
GCCGTAT97050.026.22554648-49
TCGCCGT98400.025.47946546-47
GTATCAT132150.022.65547850-51
GGTCGCC118150.021.96259144-45
TCTCGGT137250.021.79518936-37
TCGGTGG144800.021.49510638-39
GTGGTCG121050.021.08381542-43
GATCTCG167450.019.86295934-35
TAGATCT166400.018.97546432-33
ATCATTA182350.018.54065152-53
CGGTGGT140900.018.31572240-41
CTCGGTG151600.017.36752138-39
CATTAAA211050.016.78386554-55
GATCGGG27000.016.0619661
GGTGGTC183550.016.05176740-41
Produced by FastQC (version 0.11.5)