FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16217284
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT365390.22530899748687883TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATC111200.024.66512138-39
CGATGTA60550.021.57018934-35
GAAACGA136200.021.20130236-37
CGTCTGA429750.020.38446216-17
ACGTGAA144750.020.24426532-33
ACCGATG67150.019.94520632-33
CACGTCT446550.019.58659614-15
TCACGTG151500.019.34288830-31
TCTGAAC487000.018.12252218-19
CCAGTCA486800.018.09418926-27
TATGCCG292250.017.42866548-49
CGTATGC293950.017.3278746-47
CTCGTAT284950.017.25015644-45
CACACGT515250.017.04308712-13
TCGTATG294250.016.95512244-45
AAACGAT123050.016.86692436-37
ACCAATC96900.016.61544436-37
AACTCCA534850.016.57616822-23
TCTCGTA289300.016.54753942-43
CGATCTC175050.016.40079540-41
Produced by FastQC (version 0.11.5)