FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23320737
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC2511671.0770114169204859TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2182190.9357294325646741TruSeq Adapter, Index 5 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG650250.039.2599946-47
CGTATGC664150.038.47796244-45
CCGTCTT664000.038.26959650-51
TGCCGTC664350.038.21949448-49
CTCGTAT668250.037.46753742-43
ATCTCGT692150.035.779240-41
TGCTTGA761650.032.4658358-59
CTTGAAA749150.032.3970260-61
GTCTTCT849750.030.13296352-53
TCTGCTT877700.029.329756-57
AGTGATC846300.028.87538536-37
TGATCTC857600.028.66576838-39
CTTCTGC919050.028.01523454-55
ACACAGT950300.026.64193332-33
ACAGTGA975400.025.96354534-35
TGAAAAA948950.025.33319762-63
AGTCACA1012800.025.25724628-29
CCAGTCA1031800.024.90482926-27
TCACACA1066050.024.07597430-31
CTCCAGT1163850.022.17101524-25
Produced by FastQC (version 0.11.5)