FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23320737
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2055070.8812200060401179Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA58550.041.7425772
GATCGGG62850.040.321341
CGCCGTA351300.034.42074246-47
CCGTATC357150.034.02888548-49
CGTATCA341150.033.2747150-51
GCCGTAT351250.032.5850448-49
GTCGCCG373800.032.1901744-45
TCGCCGT351350.031.89003246-47
GTATCAT400300.030.35418550-51
TGGTCGC376500.030.27028842-43
GGTCGCC389700.029.36065544-45
GTGGTCG400550.027.937442-43
ATCATTA479000.027.1643652-53
TCGGTGG466900.026.1119438-39
CGGTGGT443000.025.31924840-41
CATTAAA552050.025.09060954-55
TCTCGGT472750.025.02087836-37
TATCATT475150.024.19102752-53
GATCTCG553200.023.85281434-35
CTCGGTG480550.023.79014838-39
Produced by FastQC (version 0.11.5)