FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16416550
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC8423245.1309440777751725TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG523400.31882460078396496TruSeq Adapter, Index 6 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2008900.045.2352759
AGAGCAC2037450.044.6200528
TATGCCG1106000.042.97590346-47
CCGTCTT1105300.042.759950-51
CGGAAGA2140850.042.6841134
TGCCGTC1108050.042.6311248-49
AAGAGCA2140300.042.5999647
GAAGAGC2151900.042.4254846
CGTATGC1126600.042.2540144-45
TCGGAAG2175850.042.0891533
CTCGTAT1143900.041.6250942-43
ATCGGAA2209250.041.4900052
GATCGGA2244450.040.8483961
CTTGAAA1107200.040.57983460-61
ATCTCGT1167250.040.56240540-41
TGCTTGA1129750.040.4365758-59
GCCGTCT1097300.040.27952248-49
ATGCCGT1103900.040.109346-47
CGTCTTC1114400.039.72650550-51
TCGTATG1138250.039.30182642-43
Produced by FastQC (version 0.11.5)