FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16416550
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC490040.2985036441883343Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA105000.071.2357562
GATCGGG116700.065.6392751
CGGGAGA162700.055.8614654
GAGCGGC127350.051.5784459
AGAGCGG135450.051.2270668
TCGGGAG167700.046.8660553
GTCGGGA55150.042.4260442
GAGAGCG106200.038.3883747
CGGGAAA81050.036.656624
TCGGGAA57050.036.354393
TCGGGGG300750.035.7195538-39
GCCGGAT76850.033.1944746-47
CGCCGGA60550.033.15563246-47
GGCGCCG138950.032.80725544-45
GAGGGGC273950.032.725919
AGCGGCG115650.032.48152510-11
CCGGATC72900.032.12987548-49
AGGGGCG191600.030.62878210-11
TCTCGGG127250.029.59512936-37
CGGAGAG77250.029.2441865
Produced by FastQC (version 0.11.5)