FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64598167
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC9873491.5284473938710985TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG5306900.8215248584375466TruSeq Adapter, Index 3 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT2510700.032.0021350-51
CTTGAAA2301700.031.79111160-61
TATGCCG2613900.031.22264946-47
TGCCGTC2587400.031.10177248-49
TGCTTGA2445600.030.55830258-59
CGTATGC2744250.029.91912744-45
CTCGTAT2888400.028.56534442-43
TCTGCTT2889550.028.15954856-57
GTCTTCT2877150.028.05851652-53
ATCTCGT3076700.026.78546540-41
TGAAAAA2703700.026.75162762-63
CTTCTGC3142000.025.8728754-55
GCATCTC3580700.023.17877838-39
AGGCATC3711650.022.25446936-37
TTAGGCA3782100.022.16199334-35
ACTTAGG3998200.021.03184132-33
GCCGTCT2509950.020.8213848-49
CGTCTTC2538050.020.70391550-51
ATGCCGT2574300.020.4289446-47
CTGCTTG2629150.019.77219256-57
Produced by FastQC (version 0.11.5)