FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21428959
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC720230.3361012543819791Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA116300.039.06649446-47
GTCGCCG124900.035.501944-45
CCGTATC130750.034.8760448-49
GCCGTAT117700.034.70720348-49
CGTATCA118250.034.44507250-51
TCGCCGT117250.033.8479846-47
AGAGCGT251400.033.101589
TGGTCGC129450.032.87801742-43
GGTCGCC136350.029.76847644-45
AAGAGCG282300.029.629718
ATCGGAA296900.028.2879623
AGATCGG298400.028.210891
TCTCGGT160300.028.20970736-37
GATCGGA299650.028.1241252
GTGGTCG148200.027.03563542-43
TCGGAAG312150.026.844464
TCGGTGG180200.026.08297738-39
CGGAAGA323550.025.8543345
GTATCAT178350.025.74084950-51
GATCTCG191400.025.6485134-35
Produced by FastQC (version 0.11.5)