FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60733806
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC608360.10016826543029428Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA126050.012.1750412
GATCGGG130450.011.8766041
TCGGGGG254850.08.35976738-39
TCGCCGT967450.07.411893494-95
GTCGCCG1095050.07.172900794-95
CGCCGTA897450.07.10076294-95
CGTATCA705900.07.099599494-95
GGCGCCG198350.07.052855544-45
TGGTCGC1269750.07.027770594-95
GGTCGCC1213200.06.846329794-95
GCCGTAT827650.06.76692994-95
CATTAAA902400.06.737281354-55
GTGGTCG1417450.06.68254694-95
CCGTATC803150.06.63917794-95
GCGTCGG148450.06.607914494-95
AGAGCGG268500.06.5451428
ATCATTA850700.06.42365252-53
TTAAAAA1503500.06.3531256-57
TCGGTGG1904300.06.25628894-95
CGGTGGT1728500.06.217900894-95
Produced by FastQC (version 0.11.5)