FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9504310
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA1771071.8634387977664872TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT672950.7080471912216668TruSeq Adapter, Index 18 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG303800.042.30338348-49
TGCCGTC306700.041.76472550-51
CGTATGC310800.041.43472746-47
CCGTCTT308950.041.392552-53
CTCGTAT313750.040.96937644-45
ATCTCGT322500.039.29113842-43
CCGCACA352300.036.62072836-37
GTCCGCA355100.036.2312234-35
ACGTCCG364350.035.47445332-33
CTTGAAA351850.034.9922762-63
GCACATC362400.034.9642938-39
ACATCTC369600.034.53436740-41
GTCTTCT376050.034.31564354-55
TCACGTC385800.033.5204930-31
TCTGCTT392850.032.8450558-59
TGCTTGA384350.032.5769560-61
AGTCACG398450.032.48555428-29
CTTCTGC404050.032.04942756-57
GCCGTCT305100.029.97139752-53
ATGCCGT305900.029.94654850-51
Produced by FastQC (version 0.11.5)