FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9504310
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1645011.7308042351312194Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA226200.040.79416346-47
CCGTATC229450.040.24241348-49
CGTATCA222950.040.16300250-51
GCCGTAT226450.039.8419148-49
GTCGCCG230350.039.4925344-45
TCGCCGT223100.039.32912446-47
TGGTCGC233450.038.1751242-43
ATCGGGA18450.038.0767362
GGTCGCC235900.038.03016744-45
GTATCAT249400.036.9045650-51
GATCGGG19350.036.5510371
GTGGTCG242900.036.3577142-43
TCGGTGG267950.034.83650638-39
TCTCGGT271000.034.04157336-37
CGGTGGT262000.033.85214640-41
CTCGGTG271700.033.50837338-39
ATCATTA287700.033.2395852-53
GGTGGTC276500.032.48032440-41
AGAGCGT564050.032.416279
TCATTAA287750.031.96956454-55
Produced by FastQC (version 0.11.5)