FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39573194
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC539400.13630438826848296Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA166100.021.31981546-47
CCGTATC171600.020.94042448-49
CGTATCA157900.019.80893550-51
GCCGTAT163250.019.14549448-49
GTCGCCG192650.017.90121844-45
TCGCCGT173450.017.1300446-47
TGGTCGC217100.015.15222242-43
GGTCGCC222500.013.9518144-45
GTATCAT275700.013.48140450-51
GATCGGG61500.011.8982021
GTGGTCG260700.011.73440842-43
TCGGTGG351850.010.86167738-39
ATCGGGA64750.010.4187772
ATCATTA406000.010.27778352-53
TCTCGGT361400.010.21312936-37
CGGTGGT325450.09.53127640-41
GATCTCG427300.09.52178934-35
CTCGGTG387200.08.49596838-39
CATTAAA533650.08.47348854-55
GGTGGTC438000.08.03106340-41
Produced by FastQC (version 0.11.5)