FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36282826
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1873520.5163655113303468Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA100900.037.489152
GATCGGG102650.036.9887351
CCGTATC496150.024.06425548-49
TCGGGGG245000.023.09131438-39
CGCCGTA518350.022.98206546-47
CGTATCA466950.022.9578650-51
CGGGAGA204250.022.748834
GAGCGGC151000.022.1590259
GTATCAT548500.021.82501250-51
AGAGCGG165550.021.4729218
GCCGTAT503900.021.35584848-49
ATCATTA634650.020.96820852-53
GGCGCCG123000.020.48933644-45
GTCGCCG585300.020.2843244-45
CATTAAA726550.020.1554354-55
CGCCGGA56650.019.85577846-47
TCGCCGT532350.019.7208846-47
GCCGGAT74500.017.80590246-47
CCGGATC74600.017.57774548-49
TGGTCGC635450.017.43242842-43
Produced by FastQC (version 0.11.5)