FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8223151
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT695410.8456733921096671TruSeq Adapter, Index 20 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA157820.19192156388712794TruSeq Adapter, Index 20 (97% over 44bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA168550.025.29812862-63
CCGTCTT188100.023.41932752-53
TGCTTGA189350.022.832560-61
TGCCGTC200550.021.72944350-51
TATGCCG215400.020.46322448-49
TGAAAAA207050.019.9853264-65
CGTCTTC186850.019.0768652-53
TCTGCTT235150.019.03125658-59
CTGCTTG192400.018.8523558-59
GCCGTCT191550.018.63425350-51
GTCTTCT238150.018.56773854-55
CGTATGC243100.018.15141346-47
GCTTGAA194050.017.95824460-61
TTGAAAA194400.017.64507962-63
ATGCCGT205900.017.28968448-49
CTTCTGC265700.016.93871956-57
CTCGTAT277300.015.82730844-45
TTCTGCT247650.014.89347556-57
GTATGCC244550.014.732246-47
GAAAAAA246550.013.9219864-65
Produced by FastQC (version 0.11.5)