FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8223151
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC150100.1825334351758833Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG22400.023.971151
ATCGGGA22500.022.805882
TCGGGGG45600.017.24077838-39
GGCGCCG24300.015.05175244-45
CGGGAGA48350.013.7567564
GAGCGGC38000.013.7492819
AGAGCGG43100.013.334578
CGCCGGA11750.012.73418946-47
GCCGGAT14700.012.60218546-47
CCGGATC15450.011.68266448-49
AGCGGCG26300.011.46796210-11
GCGCCGT14700.011.30975744-45
GGGCGCC42600.011.26231242-43
CATTAAA148300.010.89001554-55
GGGTCGC14600.010.7368542-43
TTAAAAA266650.010.72382256-57
AGGGGCG40700.010.56142310-11
TCTCGGG30900.010.53082536-37
CTCGGGG41850.010.38618336-37
GGGGCGC34400.010.08045742-43
Produced by FastQC (version 0.11.5)