FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33735502
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1198200.3551747947903665Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA244950.035.12828446-47
CCGTATC261350.033.30436348-49
GATCGGG77200.032.6929441
ATCGGGA75100.032.517452
GTCGCCG261800.031.83351544-45
TGGTCGC267000.028.58055742-43
CGTATCA243850.026.90967250-51
TCGCCGT239850.026.52766646-47
GCCGTAT249200.026.45580948-49
GTATCAT332550.026.12384250-51
TCGGTGG392450.023.14227538-39
GGTCGCC285300.022.8346844-45
TCTCGGT377850.022.42726536-37
GATCTCG455900.021.93703334-35
ATCATTA449450.021.6699152-53
GTGGTCG300000.021.31995842-43
AGAGCGG143250.020.6893778
CATTAAA537450.019.8628854-55
GAGCGGC145700.019.004919
GGCGCCG121900.018.72365444-45
Produced by FastQC (version 0.11.5)