FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53594035
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG153700.022.77399648-49
CGTATGC164750.021.46305846-47
CTCGTAT187600.018.93756544-45
ATCTCGT236200.015.10132242-43
TGCCGTC242850.014.46269450-51
CCGTCTT250950.014.24242752-53
CGGAATC263150.013.68215938-39
TCGTATG176300.012.93136344-45
TTCGGAA309850.012.24860236-37
GTTTCGG310100.012.13914634-35
ATGCCGT196650.011.26687148-49
TCTCGTA207200.010.88826942-43
ACGTTTC382900.09.97996632-33
GCCGTCT228950.09.843389550-51
AATCTCG238500.09.83784140-41
GTATGCC250550.09.31711546-47
TCGGAAT265500.08.89166536-37
AGTCACG440700.08.69793728-29
TCACGTT449350.08.5569630-31
CGTTTCG276550.08.53632832-33
Produced by FastQC (version 0.11.5)