FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19622744
Sequences flagged as poor quality0
Sequence length101
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCCC1873000.034.6627374-75
GCCCCCC2132300.032.41272774-75
TGGGGGG2684700.031.69165474-75
GAAAAAA1680250.031.56536174-75
CTTTTTT2243000.030.4688574-75
CAAAAAA1922450.030.46603474-75
TAAAAAA1027250.030.08413174-75
GTTTTTT1333400.028.73443274-75
AGGGGGG3116950.028.70907474-75
ACCCCCC1832500.028.22448774-75
ATTTTTT1622050.028.02259674-75
GCTTTTT538500.026.64756674-75
CGTTTTT153850.025.94885674-75
CGAAAAA139650.025.91294574-75
TACCCCC246400.025.73133574-75
CGCTTTT78900.025.54716972-73
CCTTTTT654000.025.53859374-75
CCAAAAA580550.025.2509574-75
ATCCCCC360650.025.13222574-75
GCAAAAA488450.025.06996374-75
Produced by FastQC (version 0.11.5)