FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005330857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56037014
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC67750.010.1641968
TTCCGAT78700.08.93102557
CCGATCT103150.07.09032069
GATCGGA1021150.06.17425594-95
ATCGGAA964300.05.893049794-95
AGATCGG1125050.05.834125594-95
TAGATCG177300.05.22354694-95
CAGATCG482700.05.21480694-95
TCGGAAG1004300.05.088483394-95
Produced by FastQC (version 0.11.5)