FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005330869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005330869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9447231
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAATCG7550.00201810316.93538671
TATGCCG33650.06.704508394-95
TCCGATC8900.00163576976.40363658
GCTAATA24101.6370905E-116.1230751
GCGTAAG8750.0094118125.98424771
GCGAAAA9550.00351590945.9813991
GGTTCGA9550.00351590945.9813991
GGTGAAA84400.05.9220461
GGTTCGT10500.00156352945.89357761
CGTATGC37550.05.88168192-93
TTCCGAT11356.3794234E-45.8582467
GGTCATA27703.6379788E-125.8428411
CTCGTAT42000.05.823932694-95
TCTCGTA49250.05.73811494-95
GCTATCA26603.8198777E-115.72655251
TATCTCG48950.05.724766392-93
GTAGAGA64950.05.7166341
GCGTAGA10050.0061981465.68381741
GGTCTTA26905.2750693E-115.6626881
GGTATAA26955.4569682E-115.65218261
Produced by FastQC (version 0.11.5)